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p53 responsive luciferase reporter pg13 luc  (Addgene inc)


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    Structured Review

    Addgene inc p53 responsive luciferase reporter pg13 luc
    P53 Responsive Luciferase Reporter Pg13 Luc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p53+responsive+luciferase+reporter+pg13+luc/PG13-luc+(wt+p53+binding+sites)+(Plasmid+%2316442)/pm38050059-97-10-14
    Average 93 stars, based on 83 article reviews
    p53 responsive luciferase reporter pg13 luc - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Luciferase:

    Article Title: Clinical and functional analysis of the germline TP53 p.K164E acetylation site variant.
    Article Snippet: .. Saos-2 cells were transiently co-transfected with 100 ng of the p53-responsive luciferase reporter PG13-Luc (Addgene) that contains 13 copies of the p53 DNA binding consensus site upstream from the polyoma promoter and 5.5 μg of pCMV-Neo-Bam (vector only) or 5.5 μg of pCMVNeo-Bam expressing wt p53, p.R175H, p.K164E, p.K164R or p.K164Q using Lipofectamine LTX (ThermoFisher Scientific), as previously described (Pinto et al. 2020; Pinto et al. 2022). .. Cells were harvested at 48 hours after transfection and lysed in Passive Lysis Buffer (Promega) containing Protease Inhibitor Cocktail and Halt Phosphatase Inhibitor Cocktail (ThermoFisher Scientific).

    Article Title: Clinical and functional analysis of the germline TP53 p.K164E acetylation site variant
    Article Snippet: .. Saos-2 cells were transiently cotransfected with 100 ng of the p53-responsive luciferase reporter PG13-Luc (Addgene) that contains 13 copies of the p53 DNA binding consensus site upstream of the polyoma promoter and 5.5 μg of pCMV-Neo-Bam (vector only) or 5.5 μg of pCMV-Neo-Bam expressing WT p53, p.R175H, p.K164E, p.K164R, or p.K164Q using Lipofectamine LTX (Thermo Fisher Scientific), as previously described ( , ). .. Cells were harvested at 48 h after transfection and lysed in passive lysis buffer (Promega) containing protease inhibitor cocktail and halt phosphatase inhibitor cocktail (Thermo Fisher Scientific).

    Binding Assay:

    Article Title: Clinical and functional analysis of the germline TP53 p.K164E acetylation site variant.
    Article Snippet: .. Saos-2 cells were transiently co-transfected with 100 ng of the p53-responsive luciferase reporter PG13-Luc (Addgene) that contains 13 copies of the p53 DNA binding consensus site upstream from the polyoma promoter and 5.5 μg of pCMV-Neo-Bam (vector only) or 5.5 μg of pCMVNeo-Bam expressing wt p53, p.R175H, p.K164E, p.K164R or p.K164Q using Lipofectamine LTX (ThermoFisher Scientific), as previously described (Pinto et al. 2020; Pinto et al. 2022). .. Cells were harvested at 48 hours after transfection and lysed in Passive Lysis Buffer (Promega) containing Protease Inhibitor Cocktail and Halt Phosphatase Inhibitor Cocktail (ThermoFisher Scientific).

    Article Title: Clinical and functional analysis of the germline TP53 p.K164E acetylation site variant
    Article Snippet: .. Saos-2 cells were transiently cotransfected with 100 ng of the p53-responsive luciferase reporter PG13-Luc (Addgene) that contains 13 copies of the p53 DNA binding consensus site upstream of the polyoma promoter and 5.5 μg of pCMV-Neo-Bam (vector only) or 5.5 μg of pCMV-Neo-Bam expressing WT p53, p.R175H, p.K164E, p.K164R, or p.K164Q using Lipofectamine LTX (Thermo Fisher Scientific), as previously described ( , ). .. Cells were harvested at 48 h after transfection and lysed in passive lysis buffer (Promega) containing protease inhibitor cocktail and halt phosphatase inhibitor cocktail (Thermo Fisher Scientific).

    Expressing:

    Article Title: Clinical and functional analysis of the germline TP53 p.K164E acetylation site variant.
    Article Snippet: .. Saos-2 cells were transiently co-transfected with 100 ng of the p53-responsive luciferase reporter PG13-Luc (Addgene) that contains 13 copies of the p53 DNA binding consensus site upstream from the polyoma promoter and 5.5 μg of pCMV-Neo-Bam (vector only) or 5.5 μg of pCMVNeo-Bam expressing wt p53, p.R175H, p.K164E, p.K164R or p.K164Q using Lipofectamine LTX (ThermoFisher Scientific), as previously described (Pinto et al. 2020; Pinto et al. 2022). .. Cells were harvested at 48 hours after transfection and lysed in Passive Lysis Buffer (Promega) containing Protease Inhibitor Cocktail and Halt Phosphatase Inhibitor Cocktail (ThermoFisher Scientific).

    Article Title: Clinical and functional analysis of the germline TP53 p.K164E acetylation site variant
    Article Snippet: .. Saos-2 cells were transiently cotransfected with 100 ng of the p53-responsive luciferase reporter PG13-Luc (Addgene) that contains 13 copies of the p53 DNA binding consensus site upstream of the polyoma promoter and 5.5 μg of pCMV-Neo-Bam (vector only) or 5.5 μg of pCMV-Neo-Bam expressing WT p53, p.R175H, p.K164E, p.K164R, or p.K164Q using Lipofectamine LTX (Thermo Fisher Scientific), as previously described ( , ). .. Cells were harvested at 48 h after transfection and lysed in passive lysis buffer (Promega) containing protease inhibitor cocktail and halt phosphatase inhibitor cocktail (Thermo Fisher Scientific).



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